Welcome to the Department of Biology

Department of Biology at Illinois State University
Department of Biology
photo credits: Diane Byers, Kevin Edwards

Microscopy and Image Analysis

Our school has extensive microscopy facilities and training for graduate students.

The Biological Sciences Microscopy Facility provides a range of instruments for light and electron microscopy, including a new, state-of-the-art Scanning Electron Microscope. For usage information, please contact Dr. Martha Cook.

Click here to view the raw data and full TIF figures for the publication,"Rapid phenotypic analysis of uncoated Drosophila samples with low-vacuum scanning electron microscopy" by Tardi, Cook, and Edwards (2012).

Here is a brief summary of the equipment in the Facility:

  • Scanning Electron Microscope
    • FEI Quanta 450 tungsten environmental scanning electron microscope with Oxford Inca 250 EDS. Funding by the National Science Foundation.
  • Transmission Electron Microscope
    • Zeiss EM10
  • Collection of Light Microscopes
    • Including Leica DMRBE equipped with darkfield, DIC, and phase contrast as well as fluorescence filter sets for UV, blue, green and GFP excitation
  • Confocal Microscope Funding by the National Science Foundation.
  • Leica TCS SP2

  • Pelco CPD2 critical point dryer
  • Hummer 6.2 sputter coater
  • Denton DV-502 vacuum evaporator
  • Pelco 3450 microwave oven with temperature probe and load cooler
  • Leica Ultracut UCT ultramicrotome

    Many Faculty Research Labs have other microscopes for their particular uses, including:

    Zeiss Axiovert inverted microscope w/ Narishige microinjector, needle puller and grinder

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More Scope Information

-- Leica SP2 laser scanning confocal system with three PMTs plus transmitted detector and three lasers (457-488-514/561/633nm excitations) mounted on inverted Leica DM IRE2 HC Fluo TCS 1-B microscope with 20-63x immersion objectives. Funding by the National Science Foundation. For confocal microscope usage information, please contact Dr. Kevin Edwards.

--Spinning disc confocal microscope for live cell imaging, with Yokogawa CSU-X1 scanning unit and 488 and 561 nm lasers, on Leica inverted microscope

--Stereo dissecting microscopes: Leica MZ9.5 stereomicroscope w/ plan apo objective and Leica MZ8 fluorescence stereomicroscope outfitted with a filter wheel for green and red fluorescence detection. A DFC300FX Leica cooled color low-light CCD camera mounts on either microscope for digital image collection. For dissecting microscopes usage information, please contact Dr. Kevin Edwards.